stui (New England Biolabs)
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New England Biolabs
stui
Stui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stui/StuI/pm42020383-213-20-21
Average 96 stars, based on 646 article reviews
Stui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stui/StuI/pm42020383-213-20-21
Average 96 stars, based on 646 article reviews
stui - by Bioz Stars,
2026-09
96/100 stars
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Transgenic Assay:Article Title: Synergistic engineering of Casδ nuclease for robust genome editing. Article Snippet: .. For maize stable transgenic line editing, the codon‐ optimized enCasδ was cloned to the Clone Assay:Article Title: Synergistic engineering of Casδ nuclease for robust genome editing. Article Snippet: .. For maize stable transgenic line editing, the codon‐ optimized enCasδ was cloned to the Article Title: The AltR transcription factor responds to plant thiosulfinates to regulate gene expression in a bacterial pathogen of onion Article Snippet: .. The bacterial promoter P altR , intergenic region of P. ananatis altG and altR , was synthesized as a dsDNA gblock by IDT (Table S4.) and cloned into the Article Title: Generation of ALK1 p.Gly48Glu mutant LUMCi029-A-3 for modeling Hereditary hemorrhagic telangiectasia type 2. Article Snippet: .. Clones carrying the mutation were identified by Plasmid Preparation:Article Title: Synergistic engineering of Casδ nuclease for robust genome editing. Article Snippet: .. For maize stable transgenic line editing, the codon‐ optimized enCasδ was cloned to the Article Title: An antiviral jacalin-like lectin gene contributes to nonhost resistance and host determination of potexviruses among Brassicaceae. Article Snippet: .. Those three fragments were assembled with KpnI-HF and Article Title: A human-specific RPGR isoform and a clinically approved Rho/ROCK inhibitor ameliorate defects associated with RPGR dysfunction Article Snippet: This vector contains three BbsI (New England Biolabs) cutting sites, which complicates the cloning of sgRNAs due to unintended cleavage. .. Hence, the plasmid modification was done by removing the additional BbsI site using NruI (NEB) and Construct:Article Title: U7 small nuclear RNA splice-switching therapeutics for STMN2 and UNC13A in Amyotrophic Lateral Sclerosis Article Snippet: The U7 SmOPT expression cassettes for testing in 293T cells were ordered as gene synthesis in pMK with a CMV-driven blasticidin resistance (GeneArt, Life Technologies). .. To generate the constructs targeting cryptic exons, these constructs were digested with FLAG-tag:Article Title: An antiviral jacalin-like lectin gene contributes to nonhost resistance and host determination of potexviruses among Brassicaceae. Article Snippet: .. Those three fragments were assembled with KpnI-HF and Control:Article Title: An antiviral jacalin-like lectin gene contributes to nonhost resistance and host determination of potexviruses among Brassicaceae. Article Snippet: .. Those three fragments were assembled with KpnI-HF and Synthesized:Article Title: The AltR transcription factor responds to plant thiosulfinates to regulate gene expression in a bacterial pathogen of onion Article Snippet: .. The bacterial promoter P altR , intergenic region of P. ananatis altG and altR , was synthesized as a dsDNA gblock by IDT (Table S4.) and cloned into the Extraction:Article Title: Profiling KRAS mutations in whole blood by error-corrected maximum depth sequencing. Article Snippet: .. For each sample, two replicate library preparations were performed for the forward strand, reverse strand, or both as follows: 5 μg of genomic DNA from whole blood extraction above was mixed with 5 μL of rCutsmart buffer (NEB catalogue #B6004S) and 2.5 μL of 10000U/mL Incubation:Article Title: Profiling KRAS mutations in whole blood by error-corrected maximum depth sequencing. Article Snippet: .. For each sample, two replicate library preparations were performed for the forward strand, reverse strand, or both as follows: 5 μg of genomic DNA from whole blood extraction above was mixed with 5 μL of rCutsmart buffer (NEB catalogue #B6004S) and 2.5 μL of 10000U/mL Article Title: Discovery of molecular glues that bind FKBP12 and structurally distinct targets using DNA-encoded libraries. Article Snippet: .. A 20.0 μL sample of post-selection DNA-encoded compounds was added to 56.5 μL of CutSmart buffer containing 1.0 unit of Polymerase Chain Reaction:Article Title: Discovery of molecular glues that bind FKBP12 and structurally distinct targets using DNA-encoded libraries. Article Snippet: .. A 20.0 μL sample of post-selection DNA-encoded compounds was added to 56.5 μL of CutSmart buffer containing 1.0 unit of Mutagenesis:Article Title: Generation of ALK1 p.Gly48Glu mutant LUMCi029-A-3 for modeling Hereditary hemorrhagic telangiectasia type 2. Article Snippet: .. Clones carrying the mutation were identified by Sequencing:Article Title: Generation of ALK1 p.Gly48Glu mutant LUMCi029-A-3 for modeling Hereditary hemorrhagic telangiectasia type 2. Article Snippet: .. Clones carrying the mutation were identified by Modification:Article Title: A human-specific RPGR isoform and a clinically approved Rho/ROCK inhibitor ameliorate defects associated with RPGR dysfunction Article Snippet: This vector contains three BbsI (New England Biolabs) cutting sites, which complicates the cloning of sgRNAs due to unintended cleavage. .. Hence, the plasmid modification was done by removing the additional BbsI site using NruI (NEB) and |